Cytokinin dehydrogenase precursor

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August undefined, 2024

WebJan 1, 2001 · Abstract. An enzyme degrading cytokinins with isoprenoid side chain, previously named cytokinin oxidase, was purified to near homogeneity from wheat and barley grains. New techniques were developed for the enzyme activity assay and staining on native electrophoretic gels to identify the protein. The purified wheat enzyme is a … WebMay 15, 2012 · Cytokinins (CKs) are a class of plant hormones that play a central role during plant cell growth and differentiation, thus, influencing numerous developmental programs including root growth and branching, control of apical dominance in the shoot, anthesis, seed development, leaf senescence and chloroplast development ( Kakimoto, 2003, …

Improvement of Growth, Yield, Seed Production and …

WebCytokinin dehydrogenase was confirmed to be present in the apoplast of cells. Co-staining of enzyme activity for laccase, an enzyme poised to function on the cell wall in the apoplast, in those tissues suggests a possible cooperation of the enzymes in cytokinin degradation. WebNov 27, 2015 · Cytokinins are a well-studied family of plant hormones. In the 1950s, Skoog and Miller first determined the structural formula of a cytokinin, 6-furfurylaminopurine, a substance isolated from a herring … fnaf security breach hax

Identification and characterization of the first cytokinin ...

WebSep 1, 2024 · In plants, cytokinin ribonucleosides mainly exist in forms of iPR and tZ ribonucleoside (tZR). They are considered as precursors and mainly converted to their active form via LOG after phosphorylation. The logseptuple mutants exhibited severe cytokinin-deficient phenotypes [14]. WebAug 21, 2007 · Transgenic tobacco plants overexpressing single Arabidopsis thaliana cytokinin dehydrogenase (CKX, EC 1.5.99.12) genes AtCKX1, AtCKX2, AtCKX3, AtCKX4, AtCKX5, AtCKX6, and AtCKX7 under the control of a constitutive 35S promoter were tested for CKX-enzymatic activity with varying pH, electron acceptors, and substrates. This … WebAn enzyme degrading cytokinins with isoprenoid side chain, previously named cytokinin oxidase, was purified to near homogeneity from wheat and barley grains. New techniques were developed for the enzyme activity assay and staining on native electrophoretic gels to identify the protein. green stuff that grows on roof

Identification of candidate genes for panicle length in

Gene: OsCKX5 (Os01g0775400) - Summary - Oryza_sativa

WebApr 26, 2024 · Cytokinin oxidase/dehydrogenase enzymes (CKX) catalyse the degradation of cytokinin by cleaving the active cytokinin form’s unsaturated isoprenoid … WebMar 29, 2024 · Cytokinins are one of the five major hormones families in plants and are important for their normal growth and environmental adaptability. In plants, cytokinins are mostly present as glycosides in … green stuff that grows in waterWebOct 28, 2024 · Cytokinin is a phytohormone involved in the regulation of diverse developmental and physiological processes in plants. Its potential in biotechnology and for development of higher-yield and more resilient plants has been recognized, yet the molecular mechanisms behind its action are far from understood. In this report, the roots … fnaf security breach hack

"WebOct 4, 2024 · As cytokinin is a key regulator for the maintenance of shoot apical meristem, deepening our understanding of the regulatory mechanisms of cytokinin biosynthesis and transport in response to … " - Cytokinin dehydrogenase precursor

Cytokinin dehydrogenase precursor

Tissue localization of cytokinin dehydrogenase in maize

Webendogenous cytokinin concentrations are nanomolar [15]. Enzyme based amperometric biosensors provide a simple, fast, selective, convenient and low cost analytical technique. Cytokinin dehydrogenase (CKX; EC 1.5.99.12) degrades cytokinins by oxidative cleavage of N6- side chain using FAD as the electron-donating cofactor [16–19]. WebApr 10, 2024 · Sugarcane is a complex polyploid aneuploid cash crop, and transgenic varieties are important for molecular genetic and traditional breeding approaches. Herein, the sugarcane variety ROC22 served as the receptor, the Bar gene served as a screening marker, and positive and negative fragments of the ScD27.2 gene, upstream of …

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WebJul 11, 2024 · Cytokinins constitutes a vital group of plant hormones regulating several developmental processes, including growth and cell division, and have a strong influence on grain yield. Chemically, they are the derivatives of adenine and are the most complex and diverse group of hormones affecting plant physiology. WebNational Center for Biotechnology Information

WebMay 30, 2024 · Among differentially expressed genes, a highly expressed gene in Basmati370, Os05g0374200 ( Cytokinin dehydrogenase 1 precursor) seems to be involved in accumulation of cytokinins, thus affecting the grain size.

WebJan 19, 2024 · OsGRF4 regulated two cytokinin dehydrogenase precursor genes (CKX5 and CKX1) resulting in increased cytokinin levels, which might affect the panicle traits (Sun et al. 2016). SP1 contains a conserved PTR2 domain composed of 12 transmembrane domains, which can regulate the activity of the spike meristem, leading to the short … In enzymology, a cytokinin dehydrogenase (EC 1.5.99.12) is an enzyme that catalyzes the chemical reaction N -dimethylallyladenine + electron acceptor + H2O adenine + 3-methylbut-2-enal + reduced acceptor The 3 substrates of this enzyme are cytokinin (here represented by N -dimethylallyladenine), electr…

WebJul 18, 2024 · In Arabidopsis, cytokinin suppresses LR initiation directly by acting at LR founder cells (Laplaze et al. 2007) whereas cytokinin inhibits initiation of LRs, but …

WebJul 25, 2024 · The aim of this study was to investigate the action spectrum of two urea derivatives, the 1,3-di(benzo[d]oxazol-5-yl)urea (5-BDPU) and the 1,3-di(benzo[d]oxazol-6-yl)urea (6-BDPU). In order to evaluate a possible adjuvant activity on cytokinins the compounds alone or in the simultaneous presence of different cytokinins were assayed … fnaf security breach hazard payTo identify an omnipresent FAD-dependent protein, that could be related to the cytokinin binding domain we performed a pBlast search. By application of pBlast search, we have found that on the sequence level the closest to CKX protein in Arabidopsis thalianais the FAD-linked D-2-hydroxyglutarate … See more To track the evolutionary origin of the CKXs, we have searched for the homologous sequences from the Uniprot, Pfam, and NCBI databases using the homology-based … See more Because our phylogenetic tree analysis could not answer the question regarding the origin of the cytokinin binding domain, we have analysed structural models of the CK-binding domains. … See more Domain architecture plays a key role in understanding the functionality of proteins along with their evolutionary history. To evaluate the gain … See more To understand the evolutionary history of the C-terminal cytokinin binding domain proteins the phylogenetic trees were inferred with the … See more green stuff wall insulationWebFeb 1, 2024 · The CKI1 histidine kinase specifies the female gametic precursor of the endosperm. Dev. Cell (2016) J. Kang Cytokinin transporters: GO and STOP in signaling. ... cytokinin dehydrogenase family and cytokinin profiling in developing maize plantlets in relation to the expression of cytokinin dehydrogenase genes. Plant Physiology and … green stuff that grows on rocksWebIn the past decade, the next-generation sequencing (NGS) tools were widely applied in whole genome sequencing and RNA sequencing (RNA-seq). The first whole genome sequence of the orchid Phalaenopsis equestris was published in 2015 [ 51] and the number of orchid genomes has continuously increased [ 52, 53, 54, 55, 56 ]. green stuff thread sealant sdsWebcytokinin, any of a number of plant hormones that influence growth and the stimulation of cell division. Cytokinins are synthesized in the roots and are usually derived from … fnaf security breach how old is gregoryWebJun 1, 2024 · Cytokinins maintain the growth potential (pluripotency) of shoot apical meristems, which provide stem cells for the generation of leaf primordia during the … fnaf security breach gry plWebDec 7, 2015 · Here, we show that the Lotus japonicus Ckx3 cytokinin oxidase/dehydrogenase gene is induced by Nod factor during the early phase of nodule initiation. ... Quantification was obtained using a multiple reaction-monitoring mode of selected precursor ions and the appropriate product ion. GenBank accession numbers: … green stuff that grows in ponds